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| Camargos,A.; Wohlres-Viana,S.; Costa,I.F.; Camargo,L.S.; Ferreira,J.C.; Ramos,A.A.; Oba,E.. |
| ABSTRACT The present study investigated the hormonal profile and expression of prostaglandin F2α (PGF2α), oxytocin and estrogen receptors in uterine tissues of postpartum cows treated with cloprostenol. Twenty Holstein-Zebu crossbred cows were treated with saline solution (treatment CONT) or cloprostenol (treatment CLO), both administered two and five days postpartum. Blood samples were collected on days two, seven, 14, 21 and 28 postpartum for progesterone, PGF2α metabolite (PGFM) and estradiol determination, and endometrial biopsy was performed in order to quantify the expression of oxytocin receptor (OXTR), prostaglandin F receptor (PTGFR) and estrogen receptor 1 (ERS1) genes. In the CLO treatment, expression of OXTR was reduced (P<0.05) but no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PTGFR; ESR1; Progesterone; Estradiol; RT-PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000400821 |
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| Nadergoli,Omid Kheyri; Feizi,Mohammad Ali Hosseinpour; Kafil,Hossein Samadi; Pouladi,Nasser; Hosseinzadeh,Ali; Rostamizadeh,Leila; Montazeri,Vahid; Fakhrjou,Ashraf; Sakhinia,Ebrahim; Seifi,Morteza. |
| ABSTRACT Background: Her-2 and ESR1 genes, that interact in the cell signaling pathway, are the most important molecular markers of breast cancer, which have been amplified or overexpressed in 30% and 70%, respectively. This study was performed to evaluate the gene expression levels of Her-2 and ESR1 genes in tumor cells and its adjacent normal tissue of breast cancer patients and compared them whit clinical-pathological features. Methods: In total, 80 tissue specimens from 40 patients, with an average age of 48.47 years, were examined by Real-time PCR technique, and ultimately evaluated the expression level of Her-2 and ESR1genes. The data were analyzed by REST 2009 V2.0.13 statistical software. Results: HER2 and ESR1 overexpression was identified in 19... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast Cancer; Her-2/neu; ESR1; Overexpression; Molecular marker; Real-time PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100317 |
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| Nadergoli,Omid Kheyri; Feizi,Mohammad Ali Hosseinpour; Kafil,Hossein Samadi; Pouladi,Nasser; Hosseinzadeh,Ali; Rostamizadeh,Leila; Montazeri,Vahid; Fakhrjou,Ashraf; Sakhinia,Ebrahim; Seifi,Morteza. |
| ABSTRACT Background: Her-2 and ESR1 genes, that interact in the cell signaling pathway, are the most important molecular markers of breast cancer, which have been amplified or overexpressed in 30% and 70%, respectively. This study was performed to evaluate the gene expression levels of Her-2 and ESR1 genes in tumor cells and its adjacent normal tissue of breast cancer patients and compared them whit clinical-pathological features. Methods: In total, 80 tissue specimens from 40 patients, with an average age of 48.47 years, were examined by Real-time PCR technique, and ultimately evaluated the expression level of Her-2 and ESR1genes. The data were analyzed by REST 2009 V2.0.13 statistical software. Results: HER2 and ESR1 overexpression was identified in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast Cancer; Her-2/neu; ESR1; Overexpression; Molecular marker; Real-time PCR. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100304 |
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